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. 2018 Jan 23;8:1457. doi: 10.1038/s41598-018-19702-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential scanning fluorimetry of β-amylase using different dyes and devices. (a) Melting curves obtained using 1 µM β-amylase with 10× SYPRO Orange in combination with a RT-PCR machine (data points recorded every 1 °C, but 3 °C steps shown for illustration purposes). (b) The same measurement as in a using the microcontroller based device. (c) CPM-fluorescence of 1 µM β-amylase measured with the microcontroller based device. (d) Comparison of the melting temperatures obtained from (a) (dark colours), (b) (medium colours) and c (light colours). The buffers were set to pH 5.0 (red), pH 6.0 (blue), pH 7.0 (yellow) and pH 8.0 (green).