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. 2018 Jan 23;8:1457. doi: 10.1038/s41598-018-19702-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Melting curves of E. coli complex I at different detergent concentrations and ThermoFAD melting curves of the wild-type enzyme and a variant. (a) CPM-fluorescence of 110 nM complex I in the presence of various DDM concentrations (ascending from orange to green). (b) ThermoFAD¹⁰ melting curves using 750 nM wild-type complex I (dark colours) or an equal amount of the V96P/N142M variant¹⁴ (light colours). The measurement was carried out with a RT-PCR machine (blue, data points recorded every 1 °C, but 2 °C steps shown for illustration purposes) and the microcontroller based device (red). (c and d) Show the melting temperatures obtained from (a) and (b), respectively.