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. 2018 Jan 23;9:338. doi: 10.1038/s41467-017-02501-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Biased neural coding in MO_s and V_RL/A/AL axons. a–c Coding in MO_s axon terminals projecting to the V_RL/A/AL. a GCaMP6f was expressed in MO_s neurons, and their axons were imaged in the V_RL/A/AL. b Calcium traces from two representative axons (mean ± s.e.m., cyan for the ipsiversive movement condition; magenta for the contraversive). The corresponding ROIs are shown in a. c–e Comparison between visual and motor activity for MO_s axons (c, n = 123), L5 somas (d, n = 395), and L2/3 somas (e, n = 904). f–h Histograms of the angle in polar coordinates for MO_s axons, L5 neurons, and L2/3 neurons. The distribution of the axons is more biased towards 90 degrees, indicating higher motor selectivity. Median, 25% quantile, and 75% quantile are represented as box plots, whisker length is 1.5 of the interquartile range. i–p Similar to a–h, but for V_RL/A/AL axon terminals projecting to the MO_s. V_RL/A/AL axons, n = 170; V_RL/A/AL L5 somas, n = 289; L2/3 somas, n = 913. Note skewed distribution of the axons towards 0 degrees, indicating higher visual selectivity. The histograms for L2/3 somas (h, p) are the same with those in Fig. 5h, j