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. 2017 Sep 4;37(2):187–208. doi: 10.1007/s10096-017-3089-2

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 12 — 3D bone tissue model. a–c Two-photon microscopic images of the 3D bone tissue structure under the influence of the antibiotic- and calcium-eluting micropattern: a P6 control [pure poly(D,L-lactic-co-glycolic) acid (PLGA)]; b PGR2 (6% PLGA, 2% rifampicin); c PGR2B2 (6% PLGA, 2% rifampicin, 2% biphasic calcium phosphate (BCP)). d Quantification of the volume fraction of tissue (left axis) and number of cells (right axis) of three conditions. The presence of BCP in the PGR2B2 condition substantially increased the production of mineralised ECM. The antibiotic did not adversely affect osteoblast viability and tissue formation. Adapted from Lee et al. [123] with permission from Elsevier