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MPH material was separated on a sucrose gradient. Four fractions were collected representing the sedimentation profile for whole and split virus particles for the H3N2 strain A/Wisconsin/67/05. The HA concentration for fractions 1–4 was measured by EIA (black) and SRID (grey). For EIA measurement of A/Wisconsin/67/05, mAb raised against A/Hiroshima/52/05 was used. Error bars represent ± 1 SD.
