Table 6.

Absolute quantification of proteins from three lots of the 2009 H1N1 vaccine (Novartis). rHA from A/Vietnam/1203/2004 was used as an internal standard. A known quantity of rHA from B/Brisbane/60/08 was spiked for quality control at a concentration of 60·5 μg/ml

Protein	Protein concentration, μg/ml (%CV)
	Lot 1	Lot 2	Lot 3
HA	25·5 (15·7)	25·6 (5·8)	25·5 (6·3)
NA	9·5 (9·4)	9·4 (5·3)	9·0 (5·5)
NP	3·5 (14·3)	3·1 (6·4)	1·6 (31·2)
Spiked rHA‐BrisB	64·0 (6·2)	64·5 (6·9)	70·5 (7·8)

HA, hemagglutinin; rHA, recombinant hemagglutinins.

Data shown are representative of the results of three biological replicates, each of which was obtained from three technical replicates.