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Emulsion particle size and cryo‐TEM characterization. Particle size as measured by dynamic light scattering of various emulsions stored at (A) 5°C, (B) RT, (C) 37°C, or (D) 60°C. Cryo‐TEM images of antigen‐adjuvant mixtures elucidate formulation spherical nanoparticle morphology of representative emulsions (E) rH5 + EM022, (F) rH5 + EM057, (G) rH5 + EM016. Oil droplets are indicated by black arrow; white arrows point to smaller particles indicative of protein.
