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. 2018 Jan 23;24:2. doi: 10.1186/s40409-018-0140-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Bioactivity of W. aegyptia venom primary fractions on OF1 mouse sperm motility. a Chromatogram of the analytical C18 RP-HPLC fractionation of the Egyptian snake venom. Most compounds eluted between fractions 8 and 19. Fraction numbers are indicated on top. Dashed line represents the linear ACN gradient. b Schematic diagram illustrating the sperm motility parameter (VCL) that was calculated by computer-assisted semen analysis (CASA) system. Positive fractions were those that exceeded +20% variations for VCL (fraction 11). Dashed lines indicate meaningful variations as set arbitrarily for defining potent compounds. c Mass characterization of fraction F11 components by MALDI-TOF MS. Five compounds were identified using the linear positive mode