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. 2018 Jan 23;24:2. doi: 10.1186/s40409-018-0140-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Bioactivity of purified F11 compounds. a Cation exchange chromatogram illustrating the purification of five compounds from F11 fraction (from 1 to 5 in red). The dashed line illustrates the NaCl gradient used for elution of the compounds. Minor peaks (two before compound 1 and one after compound 5) were not purified. (Inset) Secondary screening of the cation exchange compound 3 (red arrow) on sperm motility parameter VCL. b RP-HPLC purification of cation exchange compound 3. Peaks at elution time 2 min are injection artifacts. c LC-ESI-QTOF MS of compound 3. Inset illustrates the MS of m/z 1048.1010 [7+]