Table I.

Primer sequences used for PCR

Gene	Oligonucleotide Sequence		Annealing temperature (°C)	Product size (bp)	PCR efficiency
18S	Forward	5′ GTAACCCGTTGAACCCCATT 3′	57	151	1.91
	Reverse	5′ CCATCCAATCGGTAGTAGCG 3′
UBC	Forward	5′ GCGGTGAACGCCGATGATTAT 3′	56	202	1.86
	Reverse	5′ TTTGCCTTGACATTCTCGATGG 3′
YWHAZ	Forward	5′ GATGAAGCCATTGCTGAACTTG 3′	56	229	1.97
	Reverse	5′ CTATTTGTGGGACAGCATGGA 3′
COL2A1	Forward	5′ AGGGCCAGGATGTCCGGCA 3′	56	195	2.0
	Reverse	5′ GGGTCCCAGGTTCTCCATCT 3′
ACAN	Forward	5′ CAACTACCCGGCCATCC 3′	57	160	2.0
	Reverse	5′ GATGGCTCTGTAATGGAACAC 3′

UBC, ubiquitin C; YWHAZ, Tyrosine 3-monooxygenase; COL2A1, collagen type II; ACAN, aggrecan; PCR, polymerase chain reaction