Table II.
Comparison of ribonucleic acid (RNA) yield and purity isolated using two different homogenisation methods and four different RNA isolation methods for four different types of cartilaginous tissues. RNA yield, purity of A articular cartilage (AC), B meniscus (M), C nucleus pulposus (NP), and D annulus fibrosus (AF) as measured by NanoDrop. Data are presented as mean and standard deviation (sd), n = 3 (goats), n = 2 (per goat per tissue per homogenisation method per RNA isolation method)
| A | RNA isolation kit | Homogenisation method | Tissue weight (mg) | Purity |
Yield (µg RNA/g tissue) | |
|---|---|---|---|---|---|---|
| A260/280 | A260/A230 | |||||
| AC | TRIzol | Magna | 70 (23.66) | 1.28 (0.11) | 0.27 (0.09) | 14.39 (12.26) |
| Spex | 56.67 (25.82) | 1.49 (0.08) | 0.22 (0.1) | 12.79 (5.06) | ||
| Lipid | Magna | 66.67 (2.58) | 1.93 (0.15) | 0.34 (0.09) | 3.33 (1.27) | |
| Spex | 61.67 (5.16) | 1.84 (0.17) | 0.46 (0.46) | 3.92 (1.32) | ||
| Fibrous | Magna | 56.17 (9.97) | 1.94 (0.18) | 0.40 (0.05) | 3.65 (1.09) | |
| Spex | 63.33 (10.33) | 1.79 (0.1) | 0.67 (0.29) | 6.81 (3.68) | ||
| Aurum | Magna | 72 (26.78) | 1.39 (0.11) | 0.23 (0.09) | 153.6 (303.6) | |
| Spex | 86.67 (18.07) | 1.46 (0.14) | 0.26 (0.12) | 7.78 (7.21) | ||
| B | RNA isolation kit | Homogenisation method | Tissue weight (mg) | Purity |
Yield (µg RNA/g tissue) | |
| A260/280 | A260/A230 | |||||
| M | TRIzol | Magna | 85.00 (13.42) | 1.41 (0.16) | 0.33 (0.21) | 12.9 (15.5) |
| Spex | 73.33 (12.91) | 1.38 (0.17) | 0.19 (0.16) | 26.34 (28.68) | ||
| Lipid | Magna | 71.67 (5.16) | 1.85 (0.16) | 0.53 (0.41) | 3.66 (2.66) | |
| Spex | 51.67 (2.58) | 1.73 (0.07) | 0.69 (0.31) | 9.65 (1.69) | ||
| Fibrous | Magna | 71.00 (21.04) | 1.95 (0.18) | 0.47 (0.24) | 4.50 (3.58) | |
| Spex | 58.33 (2.58) | 1.66 (0.17) | 0.30 (0.29) | 4.14 (3.15) | ||
| Aurum | Magna | 88.33 (5.50) | 1.44 (0.10) | 0.49 (0.32) | 114.9 (174) | |
| Spex | 80.00 (15.49) | 1.29 (0.03) | 0.19 (0.14) | 36.39 (80.13) | ||
| C | RNA isolation kit | Homogenisation method | Tissue weight (mg) | Purity |
Yield (µg RNA/g tissue) | |
| A260/280 | A260/A230 | |||||
| AF | TRIzol | Magna | 78.33 (18.07) | 1.32 (0.22) | 0.24 (0.2) | 12.97 (6.14) |
| Spex | 83.33 (5.16) | 1.24 (0.16) | 0.12 (0.04) | 6.17 (3.95) | ||
| Lipid | Magna | 70.00 (0.00) | 1.88 (0.20) | 0.24 (0.10) | 2.16 (0.67) | |
| Spex | 68.33 (5.16) | 1.73 (0.20) | 0.44 (0.42) | 3.94 (2.00) | ||
| Fibrous | Magna | 95.17 (31.28) | 1.94 (0.20) | 0.36 (0.17) | 2.42 (0.81) | |
| Spex | 51.67 (2.58) | 1.78 (0.05) | 0.19 (0.12) | 2.65 (0.18) | ||
| Aurum | Magna | 83.83 (6.01) | 1.45 (0.09) | 0.47 (0.36) | 113.2 (79.18) | |
| Spex | 66.67 (18.07) | 1.32 (0.10) | 0.26 (0.13) | 23.6 (32.32) | ||
| D | RNA isolation kit | Homogenisation method | Tissue weight (mg) | Purity |
Yield (µg RNA/g tissue) | |
| A260/280 | A260/A230 | |||||
| NP | TRIzol | Magna | 75 (19.49) | 0.98 (0.13) | 0.13 (0.04) | 26.06 (9.8) |
| Spex | 83.33 (5.16) | 1.04 (0.08) | 0.15 (0.04 | 5.44 (2.99) | ||
| Lipid | Magna | 68.33 (14.38) | 1.49 (0.12) | 0.22 (0.20) | 3.22 (1.67) | |
| Spex | 65.00 (15.49) | 1.67 (0.13) | 0.47 (0.34) | 2.46 (0.28) | ||
| Fibrous | Magna | 86.67 (29.44) | 1.65 (0.20) | 0.46 (0.18) | 2.86 (0.83) | |
| Spex | 43.33 (12.91) | 1.76 (0.15) | 0.13 (0.08) | 4.03 (0.59) | ||
| Aurum | Magna | 64.83 (23.16) | 1.52 (0.26) | 0.8 (0.7) | 102.8 (143.9) | |
| Spex | 53.33 (20.66) | 1.36 (0.21) | 0.26 (0.11) | 43.66 (87.12) | ||