Table III.
Overview of successful analysis of gene expression
| RNA isolation kit | Homogenisation method | Articular cartilage |
Meniscus |
Annulus fibrosus |
Nucleus pulposus |
||||
|---|---|---|---|---|---|---|---|---|---|
| COL2A1 | ACAN | COL2A1 | ACAN | COL2A1 | ACAN | COL2A1 | ACAN | ||
| TRIzol | MagNA | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 2/6 | 2/6 |
| Freezer | 6/6 | 6/6 | 4/6 | 5/6 | 5/6 | 5/6 | 4/6 | 4/6 | |
| Lipid | MagNA | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 |
| Freezer | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | |
| Fibrous | MagNA | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 |
| Freezer | 6/6 | 6/6 | 2/6 | 2/6 | 6/6 | 6/6 | 6/6 | 6/6 | |
| Aurum | MagNA | 5/6 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 3/6 | 3/6 |
| Freezer | 6/6 | 6/6 | 2/6 | 2/6 | 5/6 | 5/6 | 5/6 | 5/6 | |
Real-time polymerase chain reaction analysis was performed for all samples to validate the quality of the isolated RNA. Numbers indicate the total of quantifiable samples. Samples were obtained from three goats. Per goat, two duplicate samples were run independently for both homogenisation methods and for the four different methods of RNA extraction
RNA, ribonucleic acid; COL2A1, collagen type II; ACAN, aggrecan; MagNA, MagNA Lyser; Freezer, Freezer Mill