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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Lab Chip. 2017 Sep 12;17(18):3146–3158. doi: 10.1039/c7lc00317j

Figure 2. Gross appearance, surface ultrastructure, and composition of ECM-derived membranes.

Figure 2

A. Digital photo of a COL-MAT membrane held by forceps demonstrating mechanical integrity and transparency. B. Scanning electron microscopy (SEM) visualization of collagen type I (COL) membrane surface ultrastructure, scale bar = 10 µm. Inset: Characteristic banding pattern visible in larger fibrils. C. SEM visualization of collagen type I and Matrigel composite (COL-MAT) membranes surface ultrastructure, scale bar = 10 µm. D. Immunofluorescence staining of laminin protein (green) in COL membranes demonstrates an expected absence of laminin protein. Scale bar = 200 µm. E. Immunofluorescence staining for laminin protein (green) in COL-MAT membranes shows robust incorporation of laminin within the fibrous microarchitecture (inset). Scale bar = 200 µm. F. SEM visualization of Transwell membrane surface ultrastructure showing 400 nm pores and smooth culture surfaces, scale bar = 10 µm.