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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 27;200(3):928–936. doi: 10.4049/jimmunol.1700273

Figure 1. Compounds 1D18 and 1B10 are comparable to IU1.

Figure 1

Compounds 1D18 and 1B10 were directly assessed against the selective Usp14 inhibitor IU1. A. Schematic depiction of the chemical structures of all three compounds. B. EL4 cell lysates were incubated with each compound of interest at various concentrations indicated for 60 min. Lysates were probed with Rho-Ub-PA and then resolved by SDS-PAGE to assess levels of deubiquitinating activity found throughout the cell. Usp14 is indicated in the figure based upon previous experiments (16) and Western blot of Usp14 (C). D. Analysis of DUB activity was determined by comparing Usp14 band intensity to the band intensity of a control band. E. Permeability (cm/s) was determined by passive diffusion of chemical compounds from one well to another through an artificial membrane after 6 h incubation at room temperature in the dark (* (* p < 0.01, ** p < 0.001, *** p < 0.0001, n.s. = not significant).