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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 27;200(3):928–936. doi: 10.4049/jimmunol.1700273

Figure 6. Poly-ubiquitinated protein levels are not changed upon Usp14 inhibition.

Figure 6

Poly-ubiquitinated protein levels of cells treated with either IU1, 1D18, 1B10, or cells containing the dominant negative phenotype for Usp14 were measured by western blot. A. Poly-ubiquitin staining with FK2 monoclonal mouse antibody after 2 h treatment with vehicle, IU1 (20 μM), 1D18 (5 μM), 1B10 (5 μM), or 10 μM MG-132. B. Cell lysates from WT Usp14 and DN Usp14 were untreated or incubated with MG-132 for 2 h then stained for poly-ubiquitin. Actin antibody was used as a loading control for all poly-ubiquitin western blots.