Table. Culture results for Wohlfahrtiimonas spp. from samples collected from patient with septicemia and wound myiasis and the patient’s home, Washington, USA*.
Collection batch no. | Sample no. | Specimen | Specimen description | Culture Wohlfahrtiimonas spp. |
---|---|---|---|---|
NA |
1 |
Blood |
Isolate sent to public health laboratories from admitting hospital |
Aerobic growth on blood agar (isolate 22912) |
1 |
2 |
Fly larvae (unidentified species, n ≈ 20) |
Larvae collected from underneath carpet where patient was found |
Growth on diluted homogenate on blood agar at 25°C (isolate 22913) |
1 |
3 |
House fly (Musca domestica) |
Emerged from larvae of sample no. 2 |
No growth |
1 |
4 |
Unidentified species in the family Calliphoridae |
Emerged from larvae of sample no. 2 |
No growth |
1 |
5 |
Calliphora vicina
|
Emerged from larvae of sample no. 2 |
No growth |
2 |
6 |
Green bottle fly and eggs (Lucilla sericata) |
Green bottle fly caught inside the patient’s home and laid eggs inside a sterile container |
Not cultured |
2 |
7 |
Lucilla sericata larva |
Larva obtained from the egg in batch no. 2 |
Growth on fifth wash (isolate 22914) and diluted homogenate (isolate 22915) on blood agar at 25°C |
NA | 8 | Meat and fruit | Fed to flies from samples 3–7 were extracted | No growth |
*NA, not applicable