Table. Properties and kinetics of 5 amebae species after experimental Yersinia pestis infection.

Species	Dormant state	Infection prevalence*			Infection intensity†			Intracellular survival‡					Intracellular replication
								24 h			48 h
		Mean, %	SEM		Mean, %	SEM		Mean, %	SEM		Mean, %	SEM
Acanthamoeba castellanii	Cyst	33.63	5.21		4.22	0.61		0	0		0	0	Inconclusive§
A. lenticulata	Cyst	51.66	3.17		6.41	0.43		10	11.55		0	0	No
A. polyphaga	Cyst	49.08	5.41		5.36	0.37		31.66	22.04		0	0	No
Dictyostelium discoideum	Spore	39.24	3.13		3.57	0.97		270	19.92		226.67	22.71	Yes
Vermamoeba vermiformis	Cyst	29.61	3.4		1.84	0.13		10	9.66		0	0	No

*Mean percentage of amebae containing >1 intracellular bacterium.
†Mean no. of intracellular bacteria per individual infected ameba.
‡Mean no. of surviving intracellular bacteria (relative to control) in experiments with 1 and 4 h of antibiotic drug exposure.
§We observed no replication in the intraameba replication assay, which we used to count intra-ameba bacterial colony-forming units before and after co-culture. However, we observed probable but nondefinitive mitotic bacterial replication in the TEM micrographs (Figure 4, panel A).