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. 2018 Jan 19;13:467–477. doi: 10.2147/IJN.S151110

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© 2018 Li et al. This work is published and licensed by Dove Medical Press Limited

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Flow cytometry analysis of T cell subsets.

Notes: The total mouse spleen cells were analyzed by using anti-mouse-CD3e-PE-cy5.5, anti-mouse-CD4-FITC and anti-mouse-CD8-PE for quality control. (A) The percentages of CD4⁺ (region Q3) and CD8⁺ (region Q1) T cells in the spleen of immunized mice are shown and the results are representative of three independent experiments. (B) The percentages of CD4⁺ and (C) the percentages of CD8⁺. One of three individual experiments is shown. n=4 animals per group, *p<0.05 compared with PBS or Blank control.

Abbreviations: Exo-R, exosomes from RH strain; Exo-M, exosomes from ME 49 strain; FITC, fluorescein isothiocyanate; PE, phycoerythrin.