Figure 6. Intact early homeostatic division but defective survival of CD8 T cells in absence of HVEM.

Equal numbers (5 × 10⁴) of CFSE labeled WT naïve (CD44^lo) OT-I and HVEM^−/− OT-I (Vα2⁺Vβ5⁺) cells were adoptively transferred into RAG deficient mice. (A) Day 4 post-transfer, lungs were harvested and analyzed for CFSE dilution. Histograms show WT OT-I cells in blue, HVEM^−/− OT-I cells in red and naïve in shaded gray. (B – E) Cells were harvested from lungs of RAG mice and stained for CD8, Vα2, Vβ5, CD44 and Ki67 at days 4 (B & C) and 15 (D & E) post-transfer. Experiment was performed one time with results are the mean ± SEM (n = 3 mice/group) and statistical significance as p<0.05; **, p<0.01; *.