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. Author manuscript; available in PMC: 2018 Jan 24.
Published in final edited form as: Science. 2017 Oct 20;358(6361):321–326. doi: 10.1126/science.aah5072

Fig. 4. Increased endothelial oxidative metabolism inhibits angiogenesis, and conditional Cox10 deletion rescues endothelial metabolism, angiogenesis, and cancer progression.

Fig. 4

(A) Effect of Coa6 overexpression (Coa6-GFP) on oxygen consumption rates at baseline and in the presence of oligomycin, FCCP, and antimycin A + rotenone (AA+R). n = 4 independent experiments. Error bars indicate = SD. (B and C) Immunofluorescent analysis of orthotopically co-transplanted Cntrl-GFP or Coa6-GFP endothelial cells and PC-3 tumor cells to assess in vivo vessel formation. (B) Cntrl-GFP vessels, left; Coa6-GFP vessels, right. GFP = transplanted GFP-tagged endothelial cells; CD31 = vasculature. GFP+ tip cells are indicated by red arrow heads. Scale bars, 50μm. (C) Quantification of vessel density (left) and tip cell formation (right), n = 3 mice per condition. (D to F) HPIN-stage FACS analysis of Δψ (D), vessel density (E), and prostate cancer weight (F) in cMyc, cMyc; Adrb2ecKO, cMyc; Cox10ecKO, and double cMyc; Adrb2ecKO; Cox10ecKO mice, showing restoration of glycolitic metabolism, angiogenesis, and cancer progression after co-deletion of Adrb2 and Cox10 in endothelial cells, n = 6 or 7 mice per condition. Error bars indicate SEM. *P<0.05. **P<0.01. ***P<0.001. ****P<0.0001.