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. 2018 Jan 17;24:332–341. doi: 10.12659/MSM.907782

Figure 5.

Figure 5

Ectopic expression of Cav-1 promotes ligand-induced phosphorylation of the epidermal growth factor receptor (EGFR) in SW480/pcDNA3.1 (control) and SW480/Cav-1 cells. (A) After 4-h serum starvation, cells were treated with EGF (20 nM) for 0, 5, 10, or 30 min, and evaluated by Western blotting using the following primary antibodies: anti-Cav-1, anti-phosphotyrosine (4G10 clone), anti-EGFR, anti-AKT, anti-phosphorylated AKT (p-AKT), anti-ERK1/2, anti-p-ERK1/2, and anti-β-actin (loading control). (B) The index of pY-EGFR to total EGFR in SW480/pcDNA3.1 and SW480/Cav-1 cells was calculated. (C) The index of p-ERK to total ERK in SW480/pcDNA3.1 and SW480/Cav-1 cells was calculated. (D) The index of p-AKT to total AKT in SW480/pcDNA3.1 and SW480/Cav-1 cells was calculated. * p<0.05 or ** p<0.01 versus SW480/pcDNA3.1.