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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biomaterials. 2017 Nov 20;156:194–203. doi: 10.1016/j.biomaterials.2017.11.022

Figure 3. Results of cell lysis assays using splenocytes from immunization with NY-ESO-1 formulations.

Figure 3

A) Summary of average lysis data at 100:1 effector:target ratio. HLA-A2 mice were immunized with different formulations (a–f; see Fig. 2A for schematic), and splenocytes were pulsed ex vivo in the presence of NYESO peptide. Splenocytes isolated from mice immunized with CpG-NYESO-E2 nanoparticles enhanced lysis activity toward A375, relative to free peptide and CpG. B) Cell lysis results at different effector:target ratios. HLA-A2 mice were immunized with different formulations (a–f; see Fig. 2A for schematic), and splenocytes were pulsed ex vivo in the presence of NYESO peptide. Lytic ability toward A375 cell line resulted from CpG-NYESO-E2 nanoparticles immunization is dose dependent. Data is presented as average ± S.E.M. of at least 3 independent experiments. Statistical significance was determined by two-way ANOVA followed by a Tukey's test (***p < 0.001; ****p <0.0001).