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. Author manuscript; available in PMC: 2018 Jan 24.
Published in final edited form as: Cell Rep. 2017 Dec 5;21(10):2696–2705. doi: 10.1016/j.celrep.2017.11.046

Figure 2.

Figure 2

Actin patches are dynamic. (A) A series of timelapse images of the proximal axon of a cortical neuron expressing GFP-Utr taken 30 minutes apart. (B) Merge of the image at t = 0 (purple) and t = 30 minutes (green). White puncta represent patches that have persisted, purple puncta represent patches that have disappeared or moved from that location. Green puncta represent patches that have appeared or moved to that location. (C) Similar images to those in (A) taken 1 minute apart. (D) Merge of image from (C) at t = 0 (purple) and t = 1 minute (green). (E) Merge of timelapse images of GFP-Utr at t = 0 (purple) and t = 1 to 11 minutes (green). (F) Graph of the number of patches that have persisted from t = 0 over time. Solid line corresponds to the fit of 2 exponentials, Tau 1 = 0.7 +/− 0.1 minutes, Tau 2 = 6.5 +/− 0.4 minutes, R2 = 0.9979. Roughly 40% of patches have kinetics corresponding to the shorter Tau, the rest have kinetics corresponding to the longer Tau. Results are a compilation of data from 3 neurons, 3 separate cultures. Scale bar 5 µm. See also Figure S3, Movies S3–S6.