Fig. 2.

(A) Competition ELISA, immobilized FH was pre-incubated with different antibodies, and the binding of patient's IgG was determined. (B) Binding of patient's IgG to recombinant proteins. (C) Inhibition of fluid-phase cofactor activity of FH: different mixtures of the required components for the inactivation of C3b by FI were incubated at 37°C and then an 8% SDS–PAGE electrophoresis was carried out under denaturing conditions. Well 1, C3b; Well 2, C3b + FI; Well 3, C3b + FI + FH; Well 4, C3b + FI + FH + patient's IgG.