A) Probability of survival of N2 worms to a HS (number of worms surviving the HS divided by the total number of worms) in control or subjected to RNAi of the indicated Rho genes. Mix indicates RAC-2/CED-10/MIG-2 RNAi. N ≥ 5 experiments/group.
B) F-actin visualization in the heads of N2 nematodes stained with P-R and of POSG-1::ABDmoe::mCherry worms in control or after a HS. N ≥ 30 worms. *P<0.05; **P<0.01.
C) Normalized P-R fluorescence in the heads of N2 worms in control or after a HS. Worms subjected to RHO-1 or CDC42 RNAi are indicated. N = 3 experiments/group.
D) Normalized RFP fluorescence in the heads of POSG-1::ABDmoe::mCherry worms in control or after a HS. N ≥ 3 experiments/group. *P<0.05; **P<0.01.