A) Probability of survival of OSG-1 KO worms to a HS in control or subjected to the RNAi of the indicated Rho genes. Mix indicates RAC-2/CED-10/MIG-2 RNAi. N ≥ 5 experiments/group.
B) P-R visualization of F-actin in the heads of the OSG-1 KO nematodes in the absence/presence of RHO-1 or CDC42 RNAi treatment, in control or after a HS.
C) Normalized P-R fluorescence in the heads of the OSG-1 KO nematodes in the absence/presence of RHO-1 or CDC42 RNAi treatment, in control or after a HS. Data are scaled to the fluorescence of N2 worms in control which is = 1. N ≥ 3 experiments/group. *P<0.05; **P<0.01.