Inflammatory Ligands |
|
|
LPS (LPS-EB Ultrapure; E. coli strain 0111:B4) |
Invivogen |
tlrl-3pelps |
ATP |
Invivogen |
tlrl-atp |
Flagellin from S. typhimurium |
Invivogen |
tlrl-epstfla-5 |
MSU Crystals |
Invivogen |
tlrl-msu |
dsDNA [Poly(dA:dT)] |
Invivogen |
tlrl-patn |
Anthrax LT |
Gift of Dr. Molly Hughes (UVA) |
n/a |
Pam3CSK4 |
Invivogen |
tlrl-pms |
PolyI:C |
Enzo |
ALX-746-021 |
ODN 1826 |
Invivogen |
tlrl-1826 |
Antibodies for Western Blot |
|
|
Rabbit polyclonal P-NFκB p65 (S276) (#3037) |
Cell Signaling Technologies |
3037 |
Rabbit monoclonal P-NFκB p65 (S536) (clone: 93H1) |
Cell Signaling Technologies |
3033 |
Rabbit monoclonal NFκB p65 (clone: C22B4) |
Cell Signaling Technologies |
4764 |
Rabbit monoclonal NFκB p65 XP (clone: D14E12) |
Cell Signaling Technologies |
8242 |
Mouse monoclonal β -actin (clone: BA3R) |
Thermo Fisher |
MA515739 |
Rabbit monoclonal P-IRF3 (S396) (clone: 4D4G) |
Cell Signaling Technologies |
4947 |
Rabbit monoclonal IRF3 (clone: D83B9) |
Cell Signaling Technologies |
4302 |
Anti-mouse HRP secondary |
JacksonImmuno |
111035144 (Ms) |
Anti-rabbit HRP secondary |
JacksonImmuno |
111035144 (Rb) |
Mouse monoclonal IκBα (clone: L35A5) |
Cell Signaling Technologies |
4814 |
Rabbit monoclonal LSD1 (C69G12) |
Cell Signaling Technologies |
2184 |
Rabbit monoclonal GAPDH XP (clone: D16H11) |
Cell Signaling Technologies |
5174 |
Antibodies for Flow Cytometry |
|
|
Rabbit monoclonal P-IRF3 (S396) (clone: D6O1M) |
Cell Signaling Technologies |
29047 |
F4/80 (PE-Cy7) (clone: BM8) |
eBioscience |
25480182 |
CD11b (Alexa Fluor 488) (clone: M1/70) |
eBioscience |
53011282 |
TLR4/MD-2 (PE-Cy7) (clone: MTS510) |
eBioscience |
25992482 |
TLR4 (PE) (clone: SA15-21) |
Biolegend |
145404 |
CD14 (APC) (clone: Sa2-8) |
eBioscience |
17014181 |
CD45 (APC-eFluor 780) (clone: 30-F11) |
eBioscience |
47045182 |
TruStain FcX (anti-CD16/32) |
Biolegend |
101320 |
Antibodies for Immunocytochemistry |
|
|
NFκB p65 (clone: C22B4) |
Cell Signaling Technologies |
4764 |
Anti-rabbit Alexa Fluor 488 secondary |
JacksonImmuno |
711545152 |
Chemical Reagents |
|
|
Hoechst 33342 |
Invitrogen |
H3570 |
DRAQ-5 |
Molecular Probes |
62254 |
FTY720 |
Cayman Chemical |
162359560 |
Ionomycin |
Thermo Fisher |
I24222 |
BAPTA-AM |
Thermo Fisher |
B6769 |
Fura-2-AM |
Thermo Fisher |
F1221 |
Fluo-4-AM |
Thermo Fisher |
F14201 |
Dynasore |
ApexBio |
A1605 |
EGTA-AM |
Thermo Fisher |
E1219 |
ELISA Kits |
|
|
Mouse IL-6 ELISA MAX Deluxe |
Biolegend |
431306 |
Mouse IL-1β ELISA MAX Deluxe |
Biolegend |
432606 |
Mouse TNFα ELISA MAX Deluxe |
Biolegend |
430906 |
Gene Expression Analysis Kits |
|
|
SensiMix SYBR NO-ROX Kit |
Bioline |
BIO-98020 |
ISOLATE II RNA Mini Kit |
Bioline |
BIO-52073 |
GoScript Reverse Transcriptase System |
Promega |
A5001 |