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. Author manuscript; available in PMC: 2019 Jan 16.
Published in final edited form as: Immunity. 2018 Jan 16;48(1):59–74.e5. doi: 10.1016/j.immuni.2017.11.026
REAGENT SOURCE IDENTIFIER
Inflammatory Ligands
LPS (LPS-EB Ultrapure; E. coli strain 0111:B4) Invivogen tlrl-3pelps
ATP Invivogen tlrl-atp
Flagellin from S. typhimurium Invivogen tlrl-epstfla-5
MSU Crystals Invivogen tlrl-msu
dsDNA [Poly(dA:dT)] Invivogen tlrl-patn
Anthrax LT Gift of Dr. Molly Hughes (UVA) n/a
Pam3CSK4 Invivogen tlrl-pms
PolyI:C Enzo ALX-746-021
ODN 1826 Invivogen tlrl-1826
Antibodies for Western Blot
Rabbit polyclonal P-NFκB p65 (S276) (#3037) Cell Signaling Technologies 3037
Rabbit monoclonal P-NFκB p65 (S536) (clone: 93H1) Cell Signaling Technologies 3033
Rabbit monoclonal NFκB p65 (clone: C22B4) Cell Signaling Technologies 4764
Rabbit monoclonal NFκB p65 XP (clone: D14E12) Cell Signaling Technologies 8242
Mouse monoclonal β -actin (clone: BA3R) Thermo Fisher MA515739
Rabbit monoclonal P-IRF3 (S396) (clone: 4D4G) Cell Signaling Technologies 4947
Rabbit monoclonal IRF3 (clone: D83B9) Cell Signaling Technologies 4302
Anti-mouse HRP secondary JacksonImmuno 111035144 (Ms)
Anti-rabbit HRP secondary JacksonImmuno 111035144 (Rb)
Mouse monoclonal IκBα (clone: L35A5) Cell Signaling Technologies 4814
Rabbit monoclonal LSD1 (C69G12) Cell Signaling Technologies 2184
Rabbit monoclonal GAPDH XP (clone: D16H11) Cell Signaling Technologies 5174
Antibodies for Flow Cytometry
Rabbit monoclonal P-IRF3 (S396) (clone: D6O1M) Cell Signaling Technologies 29047
F4/80 (PE-Cy7) (clone: BM8) eBioscience 25480182
CD11b (Alexa Fluor 488) (clone: M1/70) eBioscience 53011282
TLR4/MD-2 (PE-Cy7) (clone: MTS510) eBioscience 25992482
TLR4 (PE) (clone: SA15-21) Biolegend 145404
CD14 (APC) (clone: Sa2-8) eBioscience 17014181
CD45 (APC-eFluor 780) (clone: 30-F11) eBioscience 47045182
TruStain FcX (anti-CD16/32) Biolegend 101320
Antibodies for Immunocytochemistry
NFκB p65 (clone: C22B4) Cell Signaling Technologies 4764
Anti-rabbit Alexa Fluor 488 secondary JacksonImmuno 711545152
Chemical Reagents
Hoechst 33342 Invitrogen H3570
DRAQ-5 Molecular Probes 62254
FTY720 Cayman Chemical 162359560
Ionomycin Thermo Fisher I24222
BAPTA-AM Thermo Fisher B6769
Fura-2-AM Thermo Fisher F1221
Fluo-4-AM Thermo Fisher F14201
Dynasore ApexBio A1605
EGTA-AM Thermo Fisher E1219
ELISA Kits
Mouse IL-6 ELISA MAX Deluxe Biolegend 431306
Mouse IL-1β ELISA MAX Deluxe Biolegend 432606
Mouse TNFα ELISA MAX Deluxe Biolegend 430906
Gene Expression Analysis Kits
SensiMix SYBR NO-ROX Kit Bioline BIO-98020
ISOLATE II RNA Mini Kit Bioline BIO-52073
GoScript Reverse Transcriptase System Promega A5001