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. 2018 Jan 24;13(1):e0190300. doi: 10.1371/journal.pone.0190300

Fig 3. Effects of Ac-SDKP on cardiac neutrophils and macrophages in myocardial infarction.

Fig 3

Cardiac infiltration by neutrophils and macrophages was analyzed by flow cytometry at 2 days post-myocardial infarction (MI). A) Gating strategy: After gating for live cells using viability dye, total myeloid cells (CD45+ CD11b+) were gated. Macrophages were identified as F4/80+ Ly-6g- cells whereas neutrophils were identified as F4/80- Ly-6g+ cells. Using Count Bright Absolute Counting Beads (Invitrogen, Carlsbad, CA) total neutrophils (B) and macrophages (C) per heart were quantified. Ac-SDKP significantly reduced expansion of cardiac macrophage but not neutrophil populations in MI. The pro-inflammatory M1 and pro-fibrotic/reparative M2 polarization (D) was analyzed in the total macrophage (F4/80+) gate. Both, M1 (CD206-) and M2 (CD206+) were increased in MI. Ac-SDKP prevented the increase in M1 macrophages and tended to decrease M2 macrophages.