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Permeabilization of the inner membrane of P. aeruginosa ATCC 27853 as a function of contact time (min) with 12.5 μg/ml of NCP-3 and NCP-2. Viable cells are impermeable to PI and are blue due to counterstaining with 4’,6-diamidino-2-phenylindole (DAPI). Conversely, PI penetrates and stains the DNA of membrane-compromised cells (red emission).
