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. 2018 Jan 24;13(1):e0191121. doi: 10.1371/journal.pone.0191121

Table 9. Effect of week, diet and fish oil supplementation on plasma adipokine concentrations of hens fed ad libitum (Ad) or restricted (Rt) either with (Supp) or without (Crtl) fish oil supplementation (n = 8 for each conditions).

Periods
(weeks)
    ADIPOQ
(μg/mL)
NAMPT
(ng/mL)
RARRES2
(ng/mL)
0 to 39 Ad Ctrl 3.44 ± 0.20 ᵃ 150.62 ± 4.19 243.45 ± 5.61 ᵃ
Supp 3.41 ± 0.17 ᵃ 150.46 ± 4.74 238.36 ± 6.15 ᵃ
Rt Ctrl 3.21 ± 0.10 ᵃ 157.47 ± 3.95 262.39 ± 7.61 ᵇ
Supp 3.24 ± 0.17 ᵃ 147.98 ± 4.09 238.02 ± 5.60 ᵃ
P diet 0.003 0.55 0.06
supp 0.99 0.09 0.003
week < 0.0001 < 0.0001 < 0.0001
diet*supp 0.66 0.16 0.05
diet*week < 0.0001 0.18 < 0.0001
    supp*week 0.09 0.005 < 0.0001
3 to 9 Ad Ctrl 5.26 ± 0.3 123.43 ± 3.85 247.84 ± 11.73
Growing Rt Ctrl 4.25 ± 0.13 126.87 ± 2.94 229.09 ± 11.95
  P diet 0.004 0.49 0.22
9 to 18 Ad Ctrl 3.16 ± 0.10 155.15 ± 4.19 264.30 ± 8.42 ᵃ
Growing Supp 3.32 ± 0.10 156.60 ± 6.44 223.03 ± 11.79 ᵇ
Rt Ctrl 3.33 ± 0.10 155.45 ± 4.98 266.98 ± 9.59 ᵃ
Supp 3.42 ± 0.13 138.91 ± 3.34 230.13 ± 11.34 ᵇ
P diet 0.24 0.08 0.64
supp 0.25 0.12 0.0003
    diet*supp 0.77 0.07 0.83
18 to 21 Ad Ctrl 2.77 ± 0.06 154.63 ± 5.32 205.51 ± 8.51 ᵃ
Before Supp 2.80 ± 0.07 158.37 ± 8.73 258.27 ± 6.48 ᵇ
laying Rt Ctrl 2.89 ± 0.07 148.22 ± 3.72 264.88 ± 7.25 ᵇ
Supp 2.73 ± 0.07 147.99 ± 5.54 264.22 ± 5.99 ᵇ
P diet 0.68 0.17 < 0.0001
supp 0.37 0.77 0.0005
    diet*supp 0.14 0.75 0.004
21 to 39 Ad Ctrl 2.76 ± 0.15 158.63 ± 6.54 218.66 ± 5.95 ᵃ
Laying Supp 2.70 ± 0.10 156.84 ± 7.05 239.47 ± 6.93 ᵃᶜ
Rt Ctrl 2.80 ± 0.12 167.90 ± 5.85 268.53 ± 11.89 ᵇ
Supp 2.70 ± 0.09 162.28 ± 6.57 244.20 ± 6.49 ᶜ
P diet 0.89 0.26 0.001
supp 0.49 0.57 0.83
    diet*supp 0.84 0.77 0.007

Results are presented as lsmeans ± SEM. P values of the effects of diet, supplementation, week and the interaction between diet and supplementation, diet and week, and supplementation and week were considered as significant if P ≤ 0.05. Different individual (a,b, c) or group (ac) of letters with no letter in common in superscript indicate significant differences.