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. 2018 Jan 24;13(1):e0191682. doi: 10.1371/journal.pone.0191682

Fig 2. Characterization of lncR492.

Fig 2

(A) Schematic of the lncR492 locus. lncR492 (accession no. AK016992) is located within the first intron of the protein-coding gene Srrm4.

(B) Northern blot of lncR494. Increasing amounts of total RNA were loaded. Black arrow indicates lncR492-specific signal at ~1400 bp. A probe targeting Gapdh mRNA was used as loading control.

(C) Analysis of polyadenylation. mRNA was transcribed into cDNA by using Oligo(dT) primer followed by PCR.

(D) RT-PCR analysis of lncR492 and Gapdh expression after the RNA extract was treated with a 5’-phosphate-dependent exonuclease, which results in a degradation of f.ex. ribosomal RNA (left panel).

(E) RNA FISH of lncR492 expression in undifferentiated ESC. The lncR492-specific signal (red) was reduced after lncR492 knock-down. Scale bars are 10 μm.

(F) Cellular fractionation in ESC was followed by RNA isolation and mRNA expression analysis by qRT-PCR.