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. 2017 Dec;109(6):579–589. doi: 10.5935/abc.20170176

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Experimental study design. (A) Animals were treated with vehicle and streptozotocin (STZ) at week 0. Bone marrow cells were harvested on the 4th week for culture and in vitro analyses. (B) MSC therapy scheme. Diabetic animals (STZ) were divided into two groups: the first one received 5 x 106 cells from healthy rats (DM + MSCc) and the second received the same quantity of cells from diabetic rats (DM + MSCd) after 4 weeks of disease. The cells were injected into the animals’ retroocular plexus. Blood glucose levels and body weight were evaluated during 4 weeks after transplantation. At the end of the protocol, the animals were sacrificed, and their hearts were isolated for potential recording.