Figure 3.

Adipogenic and osteogenic differentiation assays. The adipogenic and osteogenic differentiation assays were performed during 21 days using MSCc and MSCd. We started the protocol with 60% confluence culture. (A) In the adipogenic assay, small lipid droplets were present in both MSCc and MSCd at day 7 and increased in volume and number on day 14. At day 21, the cultures were stained with oil red O, and the lipid droplets were stained in red. Both MSCc and MSCd differentiated into adipocytes at the end of the protocol. (B) In the osteogenic assay, we observed dramatic morphologic changes starting at day 7 in both MSCc and MSCd, accentuating at day 14. At day 21, the cultures were stained with alizarin red and the calcium deposits stained in red. Both MSCc and MSCd generated a mineralized matrix at the end of the protocol.