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SAVICs undergo calcification in vitro. Calcium deposition determined by HCl leaching (µg/mg protein) in VICs treated with (A) calcium (Ca) alone (1.5–5.4 mM) and phosphate (Pi) alone (1.5–5.0 mM), and (B) calcium and phosphate in combination (1.5 to 3.6 mM Ca/0.95 to 2.5 mM Pi). Results are presented as mean ± SEM. **P<0.01; ***P<0.001 compared with control; n=6.
