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Upregulation of osteogenic gene marker expression in calcifying SAVICs. Fold change in the mRNA expression of (A) RUNX2, (B) PIT1, and (C) MGP in sheep aortic valve interstitial cell line (SAVIC) treated with Ca (2.7–5.4 mM) for 48 h. Results are presented as mean ± SEM. *P<0.05, **P<0.01; ***P<0.001 compared to control; n=6.
