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. 2017 Nov 23;17(2):2113–2120. doi: 10.3892/mmr.2017.8145

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-29b may directly target DNMT3b. PANC-1 cells were transfected with miR-29b mimics or inhibitor, and the (A) mRNA and (B) protein levels of DNMT3b were determined. (C) Relative expression of miR-29b was measured using reverse transcription-quantitative polymerase chain reaction. (D) The predicted target sites of miR-29b. (E) The luciferase reporter assay was performed to confirm the direct target sites. **P<0.01, ***P<0.001 vs. NC. Data are presented as the mean ± standard deviation. DNMT3b, DNA methyltransferase 3b; miR, microRNA; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutant.