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. 2017 Nov 23;17(2):2113–2120. doi: 10.3892/mmr.2017.8145

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — DNMT3b is able to reverse miR-29b-induced apoptosis. (A) Relative expression of DNMT3b mRNA in cells was measured using reverse transcription-quantitative polymerase chain reaction. (B) PANC-1 cells were cotransfected with miR-29b inhibitor and NC siRNA, miR-29b inhibitor and DNMT3b siRNA, miR-29b mimics and vector control, miR-29b mimics and overexpression DNMT3b plasmid. Cell viability was analyzed, and apoptosis was (C) quantified following (D) flow cytometry analysis in PANC-1 cells transfected with miR-29b mimics or inhibitor and si-DNMT3b or negative control. (E) Hoechst staining was performed to confirm the results of the viability and apoptosis assays. *P<0.05 vs. inhibitor; ^#P<0.05 vs. mimics. Data are presented as the mean ± standard deviation. miR, microRNA; OD, optical density; siRNA, small interfering RNA; DNMT3b, DNA methyltransferase 3b; PI, propidium iodide; FITC, fluorescein isothiocyanate.