Figure 1.

Minimal murine Hcrt gene promoter and its synthetic derivatives. Modified DNA sequences were generated based on the proximal 5′ upstream region (~-390bp) of the mouse Hcrt gene, which included the complete proximal OE1 element (blue box). The modifications included deletions or mutations of olf-1 sites (red boxes) proximal (M1/D1) or distal (M2/D2) to the putative TSS of the Hcrt mRNA (indicated by an arrow). The TATAA box is outlined by a black box, whereas the initial ATG codon of Hcrt is highlighted by a light-orange box. Green boxes outline restriction enzyme sites added for cloning purposes. A second probable TSS was identified, indicated by a question mark above the arrow. TSS, transcription start site; WT, wild-type; D, deletion; M, mutation.