Figure 3.

Expression of a luciferase reporter gene is driven by different derivatives of the minimal Hcrt promoter in semi-stable 293-ebf2 cells. Luciferase signals from semi-stable 293-ebf2 cells show the expression of luciferase driven by the different Hcrt promoter sequence derivatives. Mutations introduced to the proximal olf-1 site (D1 and M1) increased luciferase, whereas mutations of the distal olf-1 site (D2 and M2) decreased luciferase, compared with that in the WT promoter sequence. *P<0.01 (analysis of variance and Holm-Sidak post-hoc test) vs. groups indicated by joining lines. n=18 experiments/group. WT, wild-type; D, deletion; M, mutation; ebf2, early B-cell factor 2.