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. 2017 Nov 27;17(2):2393–2401. doi: 10.3892/mmr.2017.8164

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Copyright: © Ding et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — COUP-TFII is downregulated in gastric cancer tissues and gastric cancer cell lines. (A) Difference in the relative expression between all ANT and tumor samples determined by RT-qPCR. (B) Western blotting analysis of 19 cases reveals the differential expression of ANT and tumor samples. Yellow arrows represent the labeling of the correct size of band. (C) Immunohistochemical analysis demonstrates the decreased expression of COUP-TFII in gastric cancer tissues (11/19) compared with normal gastric mucosa tissues; scale bar, 200 µm. (D) Expression levels of COUP-TFII mRNA and protein in three gastric cancer cell lines (SGC-7901, SGC-823, MGC-803) as well as the GES-1 cell line. ***P<0.001. ANT, adjacent non-neoplastic tissues; COUP-TFII, chicken ovalbumin upstream promoter transcription factor 2; N, normal; T, tumor.