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. 2017 Dec 5;17(2):2853–2860. doi: 10.3892/mmr.2017.8192

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of siRNA-WWTR1 on expression of WWTR1 downstream factors. (A) After siRNA-WWTR1 transfection for 48 h, the mRNA expression of ASNS, ID1, SMAD3 and BTC were measured by RT-PCR. (B and C) ASNS, ID1, SMAD3 and BTC protein expression were determined by western blotting after siRNA-WWTR1 transfection for 48 h. GAPDH was also detected as the control of sample loading. Data were presented as mean ± SD, n=6, **P<0.01 vs. control; ^##P<0.01 vs. mock. WWTR1, WW-domain containing transcription regulator 1; ASNS, asparagine synthetase (glutamine-hydrolyzing); ID1, inhibitor of DNA binding 1, HLH protein; SMAD3, mothers against decapentaplegic homolog family member 3; BTC, betacellulin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.