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. 2017 Dec 11;17(2):2957–2963. doi: 10.3892/mmr.2017.8249

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — NSP can restore disruption of autophagic flux after SCI. (A) Western blot analyses of LC3 and p62 protein in the spinal cord at 72 h after SCI. (B) Quantitative analysis of western blot analyses indicated that NSP reduces LC3-II and p62 protein levels at 72 h after SCI. Mean ± SD (n=6/group). *P<0.05, **P<0.01 compared with the vehicle-group. (C) Representative confocal images of LC3 and MAP-2 immunofluorescence double staining in the sham group, NSP-treated and vehicle-treated animals. Vehicle-treated rats exhibited increased dots of LC3 compared with the sham control at 72 h after SCI. NSP decreased the dots of LC3 vs. vehicle control (n=3/group; scale bars, 50 µm).