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. 2017 Dec 12;17(2):3035–3041. doi: 10.3892/mmr.2017.8262

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Copyright: © Pang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Representative photomicrographs demonstrating spleen sections the (A-C) VC (dimethyl sulfoxide), (D-F) positive (GTN) and (G-I) BetA-treated groups on days 4, 8 and 12 after treatment. Inset (IS) is representative of the area in the figure outlined in black. Black arrows identify the NADPH-d-stained blood vessels (BV), the red arrow points the perivascular nerve fibres and blue arrow demonstrates the NADPH-d positive cells in the red pulp (RP) region. ‘WP’ indicates the white pulp region. Scale bar, 100 µm. (J) The intensity (OD) of the NADPH-d staining was quantified using Olympus Soft Imaging cellSens software version 1.6. Data are expressed as means ± standard deviation of mice (n=6 per group) **P<0.001 vs. the VC group, ^#P<0.001 vs. the GTN group, ^†P<0.01 vs. the VC group. GTN, goniothalamin; BetA, betulinic acid; NADPH-d, nicotinamide adenine dinucleotide phosphate diaphorase; VC, vehicle control; OD, optical density.