Table 2.
Program/language | Version | Application | Execution order |
---|---|---|---|
Bowtie2 | 2.2.4 | Maps contigs to reference, creating an SAM file (Langmead and Salzberg 2012) |
1 |
SAMtools | 1.2 | Converts SAM to BAM (Li 2011; Li et al. 2009) | 2 |
BEDTools | 2.17.0 | Converts BAM to BED (Quinlan and Hall 2010) | 3 |
Python (BioPython) |
2.7.3 1.66 |
Executes the U50 program (Cock et al. 2009; Lutz 2013) | 4 |
QUAST | 2.3 | Calculates assembly metrics (N50, N75, L50, GC%, etc.) based on the input contig file (Gurevich et al. 2013) | 5 |