Figure 5.

The FasL/Fas ratio is inversely correlated with the invasive abilities of OSCC cells and significant antimetastatic effects of the hcc49scFv-FasL in a SAS orthotopic graft model. A, In vitro invasive abilities of OSCC cell lines. Values are presented as the mean ± SE of three independent experiments. Data were analyzed using a one-way ANOVA with Tukey post hoc tests at 95% confidence intervals; different letters represent different levels of significance. B, Correlation between the FasL/Fas ratio and the invasiveness of the OSCC cell lines. Spearman correlation coefficient = 0.9; P = 0.037. C, SAS cells were treated by the 50 μg/mL hcc49scFv-FasL for the Matrigel invasion assays. Values are presented as the mean ± SE of three independent experiments. *, P < 0.05 compared with the vehicle groups. D, Mesenchymal markers, N-cadherin, and Snail were expressed in hcc49scFv-FasL–treated SAS cells. Lysates were collected from cells cultured with or without hcc49scFv-FasL (50 μg/mL) for 24 hours and subjected to a Western blot analysis. E, Administration of the hcc49scFv-FasL through an intraperitoneal injection prevented the neck lymph node metastasis of DsRed-labeled SAS cells. F, Neck lymph node tissues were histologic analyzed by H&E staining. Histopathology of the lymph nodes are shown at ×100 (top) and ×400 (bottom). T, tumor part; NT, nontumor part. G, An overall survival curve was produced for tumor-bearing mice after treatment without or with the hcc49scFv-FasL using the Kaplan–Meier method. The P values were determined using a log-rank test.