Figure 3.

lncRNA-XIST regulates autophagy through ATG7. (A) A549 cells were transfected with Sh-X or the control plasmid (Ctrl) for 72 h, and the expression of ATG7, ATG5, ATG4 and BECN1 was examined by western blotting. GAPDH was used as the normalization control. (B) A549 cells were co-transfected with Sh-X and ATG7 plasmid or control plasmid for 72 h, and the LC3B-II accumulation and SQSTM1 degradation were detected by western blotting. (C) The rate of LC3BII/GAPDH (autophagic flux) is shown. (D and E) A549 cells were co-transfected with Sh-X, GFP-LC3B plasmid and ATG7 plasmid for 72 h and the number of GFP-LC3B dots were examined by (D) confocal microscopy and (E) quantified for each cell. Data are shown as mean ± SD of three independent experiments. *P<0.05; **P<0.01.