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. 2017 Oct 23;38(6):3355–3366. doi: 10.3892/or.2017.6052

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Copyright: © Hao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Role of c-Fos in the regulation of FUT1. (A) The activity of human FUT1 promoter construct in 293 cells and ovarian cancer cells lines was detected. (B) The luciferase reporter assay of FUT1 promoter constructs in 293 cells and ovarian cancer cells. Cell lines 293, SKOV3, CAVO3 and ES-2 were co-transfected with pGL4-FUT1, with empty vectors, or pCMV6-c-Jun or pcDNA3-c-Fos or both. (C) ChIP assay detected c-Fos and c-Jun interacted with FUT1 promoter in CAVO3 cells. Data represent the mean ± SD of 3 independent experiments performed in triplicate; *P<0.05.