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. 2017 Oct 23;38(6):3355–3366. doi: 10.3892/or.2017.6052

Figure 5.

Figure 5.

TGF-β1 activates c-Fos through the MAPK/AP-1 pathway. Upon TGF-β1 treatment, western blotting was employed to determine c-Jun and c-Fos expression in 3 ovarian cells. (A) SKOV3, (B) CAVO3, (C) ES-2. c-Fos and c-Jun expression is related to TGF-β1 in a concentration manner in ovarian cells pretreated with various concentrations of TGF-β1 (0.2, 1, 2, 5 and 10 ng/ml) for 5 min. (D) Western blot analysis was carried out to determine the expression of c-Fos, Lewis y and the downstream signal elements of the MAPK signaling pathway, JNK, p38 and ERK, and the change in the phosphorylation level of JNK, p38 and ERK. CAVO3 cells were treated with pcDNA-c-Fos, c-Fos siRNA, inhibitor of TGF-β1 receptor (LY2109761) and JNK (SPF600125) upon TGF-β1 stimulation.