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. 2017 Oct 23;38(6):3355–3366. doi: 10.3892/or.2017.6052

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Copyright: © Hao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — TGF-β1 activates c-Fos through the MAPK/AP-1 pathway. Upon TGF-β1 treatment, western blotting was employed to determine c-Jun and c-Fos expression in 3 ovarian cells. (A) SKOV3, (B) CAVO3, (C) ES-2. c-Fos and c-Jun expression is related to TGF-β1 in a concentration manner in ovarian cells pretreated with various concentrations of TGF-β1 (0.2, 1, 2, 5 and 10 ng/ml) for 5 min. (D) Western blot analysis was carried out to determine the expression of c-Fos, Lewis y and the downstream signal elements of the MAPK signaling pathway, JNK, p38 and ERK, and the change in the phosphorylation level of JNK, p38 and ERK. CAVO3 cells were treated with pcDNA-c-Fos, c-Fos siRNA, inhibitor of TGF-β1 receptor (LY2109761) and JNK (SPF600125) upon TGF-β1 stimulation.