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Representative immunostainings for HMGB1 and calreticulin in breast cancer (A) and ESCC (B) cases using anti-HMGB1, anti-calreticulin mAbs. The grade of HMGB1 and calreticulin expression was scored as 0 (0–10% positive), 1+ (>10–30% positive), 2+ (>30–80% positive), or 3+ (>80% positive) based on the tumor cells. Sections in individual sample were observed at a magnification of ×400. HMGB1, high-mobility group box 1 protein; ESCC, esophageal squamous cell carcinoma.
