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. 2017 Nov 14;39(1):151–159. doi: 10.3892/or.2017.6097

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Copyright: © Aoto et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — In vitro treatment of breast cancer cell lines with chemotherapeutic drugs. (A) The proportion of dying cells [Annexin V(+) or 7-AAD(+)] was analyzed by flow cytometry. (B) HMGB1 in the supernatant after chemotherapy was evaluated by ELISA. (C) Calreticulin expressed on the cell surface after chemotherapy was evaluated by flow cytometry. Representative histograms for these cell lines are shown. *p<0.05, **p<0.001. PTX, paclitaxel; DXR, doxorubicin; HMGB1, high-mobility group box 1 protein. n.s., not significant.